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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in many tumors, which leads to chromosome instability, and therefore may contribute to tumorigenesis. This protein was discovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital role in marketing mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are already described. Two added splice variants had been claimed but thorough nucleotide sequence info will not be nonetheless accessible.
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Blocking peptides are peptides that bind specifically towards the goal antibody and block antibody binding. These peptide ordinarily has the epitope acknowledged with the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This guideline outlines further more probable optimization of mobile and immunoassay parameters to ensure the very best success are...
Antibodies sure to the blocking peptide now not bind to your epitope over the concentrate on protein. By evaluating the staining through the blocked antibody as opposed to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic occasion. Cyclin E forms a posh with and functions as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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